A gene coding for an aldehyde/alcohol dehydrogenase in C. acetobutylicum! (in C. beijerinckii is missing -> activates ethanol production?) see Molecular characterization of an aldehyde/alcohol dehydrogenase gene from Clostridium acetobutylicum ATCC 824: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC205125/

PTS converts carbohydrates into phosphoesters; containing genes: ptsI, ptsH, hprK, manIIAB, manIIC (X276_23240, X276_02985), manIID (X276_23235, X276_02990), manIIB, manIIA

σ factor; family sigma 70; GenBank locus tag in reference sequence NZ_CP011966.2: X276_22455

butyryl-CoA dehydrogenase; K00248 ; KEGG: Cbei_0322

Lactate production occurs only when ferredoxin and hydrogenase level is reduced

alcohol dehydrogenase B; KEGG: Cbei_1722

Phosphate acetyltransferase; X276_20710; Cbei_1164

3-hydroxybutyryl-CoA dehydrogenase; K00074; KEGG: Cbei_0325

scoB; CoA transferase subunit B; part of the sol operon; K01029; KEGG: Cbei_3834, Cbei_2653

butanol dehydrogenase; K00100 Source: https://www.genome.jp/kegg-bin/show_pathway?org_name=cbe&mapno=00650&mapscale=1.0&show_description=hide

sporulation stage 0 transcription factor. GenBank locus tag in reference sequence NZ_CP011966.2: X276_RS08400

Butyrate (acid) producer. GenBank locus tag in reference sequence NZ_CP011966.2: X276_RS01200; KEGG: Cbei_0204, Cbei_4006, Cbei_4609

sporulation sigma factor SigF; GenBank locus tag in reference sequence NZ_CP011966.2: X276_22650

alcohol dehydrogenase A; KEGG: Cbei_2181

GenBank locus tag in reference sequence NZ_CP011966.2: X276_20950

Butyryl phosphate

GenBank locus tag in reference sequence NZ_CP011966.2: X276_20920

phosphate butyryltransferase; GenBank locus tag in reference sequence NZ_CP011966.2: X276_25645; Cbei_4899

Butyralaldehyde

https://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY-6594

Genes for the enzymes involved in the subsequent C-3 part of glycolysis

aldehyde dehydrogenase, butanol (solvent) producer; part of the sol operon. GenBank locus tag in reference sequence NZ_CP011966.2: X276_RS20360; KEGG: Cbei_3832; key enzyme in the formation of butyraldehyde from butyryl-CoA; corresponding to adhE1 in C. acetobutylicum

pyruvate kinase; X276_01375

enoyl-CoA hydratase; K01715; KEGG: Cbei_0322

butanoyl-CoA

n-butanoate https://biocyc.org/META/NEW-IMAGE?type=PATHWAY&object=PWY-6594

Gene encodes a member of the glucose phosphate isomerase protein family. X276_25070

complex of genes: X276_14415, 14410, 14405, 14400, 14395, 14390, 14385

acetate kinase; X276_20705; Cbei_1165

phosphorylated sporulation stage 0 transcription factor.

electron-transferring flavoprotein complex

GenBank locus tag in reference sequence NZ_CP011966.2: X276_22660

spoIIAA is dephophorylated by SPOIIAB -> unfunction; spoIIE phosphorylates spoIIAA and spoIIAA inactivated spoIIAB

phosphorylaion spoIIA by spoIIAB

GenBank locus tag in reference sequence NZ_CP011966.2: X276_01205

sporulation sigma factor SigE

6-phosphofructokinase; X276_01370

thiolase, acetyl-CoA acetyltransferase; K00626; KEGG: Cbei_0411

fructose bisphosphate aldolase; X276_17335 and X276_11155; KEGG: Cbei_1903

acetoacetate decarboxylase; part of the sol operon; KEGG: Cbei_3835

It seemed that butanol could disrupt fluidity and function of the cell membrane. Destroyed cell membrane causes cell death

anti-sigma F factor; Cbei_0813; GenBank locus tag in reference sequence NZ_CP011966.2: X276_22655

CoA transferase subunit A; part of the sol operon; KEGG: Cbei_3833, Cbei_2654

Pyruvate is the product of glucose. Solvents (ABE) inhibit glucose uptake (due to the restriction of CellCollective inhibition of pyruvate instead of glucose)