[, label=Opisthokonta, dot
    [, tier=1
        [Nucleariida]
        [Fungi]
    ]
    [, label=Holozoa, dot
        [Filasterea]
        [Ichthosporea]
        [, tier=1
            [\color{red}{Animals}]
            [Choanoflagellata]
        ]
    ]
]