casgen
| Crates.io | casgen |
| lib.rs | casgen |
| version | 0.1.4 |
| source | src |
| created_at | 2022-10-08 01:20:43.241147 |
| updated_at | 2022-10-08 17:17:51.390833 |
| description | A way to generate cas12 6-plex CRISPR sequences |
| homepage | |
| repository | https://github.com/noamteyssier/casgen |
| max_upload_size | |
| id | 683228 |
| size | 18,806 |
Noam Teyssier (noamteyssier)
documentation
README
casgen
Generates randomized CAS12 style 6-plex CRISPR reads.
Construct
A construct is defined by the following structure:
{stagger}{left_adapter}
[{constant}{spacer}] *6
{right_adapter}{stagger}
Where the stagger is a random sequence of 0-8 basepairs,
the {left,right}_adapter is not guaranteed to be the
same length.
The constant region will be the same length and the spacer
region is the same length as well.
Usage
Installation
cargo install casgen
Running
casgen
Running with Customizations
Check out the help menu
casgen --help